Cardiff Blues stars Alex Cuthbert, Cory Allen, Matthew Rees and Josh Turnbull will all represent Wales in their opening game in South Africa against Eastern Province Kings in Port Elizabeth on Tuesday (kick-off 7pm local time).
Warren Gatland's starting XV includes four uncapped players, with Matthew Morgan lining up at full-back, Steven Shingler at inside centre and Jordan Williams on the wing.
They are joined in the backline by Cardiff Blues duo Alex Cuthbert and Cory Allen as well as James Hook and Rhodri Williams at half back.
In the pack Paul James is named alongside Scott Baldwin and Rhodri Jones in the front-row with Jake Ball and Ian Evans in the second-row.
Captain Dan Lydiate is joined by new Cardiff Blues back row Josh Turnbull at flanker with Dan Baker at No 8.
On the bench Cardiff Blues hooker Matthew Rees is named in a Wales squad for the first time since February 2013.
Uncapped Gareth Davies is named amongst the replacements along with George North and Liam Williams.
WALES TEAM TO PLAY EASTERN PROVINCE KINGS:
Matthew Morgan (Ospreys), Alex Cuthbert (Cardiff Blues), Cory Allen (Cardiff Blues), Steven Shingler (Scarlets), Jordan Williams (Scarlets), James Hook (Perpignan), Rhodri Williams (Scarlets), Paul James (Bath), Scott Baldwin (Ospreys), Rhodri Jones (Scarlets), Jake Ball (Scarlets), Ian Evans (Ospreys), Dan Lydiate (Capt) (Racing Metro), Josh Turnbull (Cardiff Blues), Dan Baker (Ospreys).
Replacements: Matthew Rees (Cardiff Blues), Aaron Jarvis (Ospreys), Samson Lee (Scarlets), Luke Charteris (Perpignan), Aaron Shingler (Scarlets), Gareth Davies (Scarlets), George North (Northampton Saints), Liam Williams (Scarlets).
Cardiff Blues travel to Myreside on Friday to take on Edinburgh Rugby in a crucial Guinness PRO12 clash, and with Wales facing Scotland in the RBS 6 Nations at BT Murrayfield on Saturday, it promises to be a weekend of non-stop rugby action at the Scottish capital. Here are some useful advice to Cardiff Blues travelling supporters.
To celebrate the opening of the newly refurbished Guinness PRO12 Pack Bar, the world famous Irish brewer will be offering supporters free pints of the black stuff ahead of Saturday's clash with Ulster.
'TACKLE' - the first-ever joint community rugby initiative between the four professional teams in Wales has been launched (today Weds 19th October) bringing an inspiring new rugby education programme to young people in Communities First areas. Partners in the project were joined by Wales' star rugby internationals Sam Warburton (Cardiff Blues), Alun Wyn Jones (Ospreys), Gareth Davies (Scarlets) and Hallam Amos (Newport Gwent Dragons), for the launch at Aberdare Community School.
A last minute Jarrod Evans penalty ensured Cardiff Blues Premiership Select finished their British and Irish Cup campaign on a high, with a 24-21 victory over London Scottish at Athletic Ground on Friday.