Cardiff Blues stars Alex Cuthbert, Cory Allen, Matthew Rees and Josh Turnbull will all represent Wales in their opening game in South Africa against Eastern Province Kings in Port Elizabeth on Tuesday (kick-off 7pm local time).
Warren Gatland's starting XV includes four uncapped players, with Matthew Morgan lining up at full-back, Steven Shingler at inside centre and Jordan Williams on the wing.
They are joined in the backline by Cardiff Blues duo Alex Cuthbert and Cory Allen as well as James Hook and Rhodri Williams at half back.
In the pack Paul James is named alongside Scott Baldwin and Rhodri Jones in the front-row with Jake Ball and Ian Evans in the second-row.
Captain Dan Lydiate is joined by new Cardiff Blues back row Josh Turnbull at flanker with Dan Baker at No 8.
On the bench Cardiff Blues hooker Matthew Rees is named in a Wales squad for the first time since February 2013.
Uncapped Gareth Davies is named amongst the replacements along with George North and Liam Williams.
WALES TEAM TO PLAY EASTERN PROVINCE KINGS:
Matthew Morgan (Ospreys), Alex Cuthbert (Cardiff Blues), Cory Allen (Cardiff Blues), Steven Shingler (Scarlets), Jordan Williams (Scarlets), James Hook (Perpignan), Rhodri Williams (Scarlets), Paul James (Bath), Scott Baldwin (Ospreys), Rhodri Jones (Scarlets), Jake Ball (Scarlets), Ian Evans (Ospreys), Dan Lydiate (Capt) (Racing Metro), Josh Turnbull (Cardiff Blues), Dan Baker (Ospreys).
Replacements: Matthew Rees (Cardiff Blues), Aaron Jarvis (Ospreys), Samson Lee (Scarlets), Luke Charteris (Perpignan), Aaron Shingler (Scarlets), Gareth Davies (Scarlets), George North (Northampton Saints), Liam Williams (Scarlets).
Cardiff Blues were crowned the Welsh champions of the Premiership Rugby 7s Series on Saturday night to book a place at the Series Final on Friday, August 8 - and you can join them with tickets on sale NOW!
Wales will tonight (Saturday) stage the first leg of the Premiership Rugby 7s Series this Saturday evening at the BT Sport Cardiff Arms Park - and tickets remain on throughout the day and right up until kick-off so you can join us for an summer night of top class rugby!
The Welsh stage of the Premiership Rugby 7s Series explodes into life at BT Sport Cardiff Arms Park this Saturday - and fans attending a night of thrilling regional rugby will also be showing their support for the #StayStrongForOws campaign.
In its sixth year the Blues Cup once again showed to be huge hit throughout the region and is a great advert for the community game as each of the six finalists across the three age groups produced rugby of an outstanding level resulting in 14 tries.
Inspired Cardiff Blues enjoyed Judgement Day at the Millennium Stadium as they secured a tense 17-13 win over regional rivals Scarlets in a feisty and enthralling encounter in the RaboDirect PRO12 on Easter Sunday.