Cardiff Blues stars Alex Cuthbert, Cory Allen, Matthew Rees and Josh Turnbull will all represent Wales in their opening game in South Africa against Eastern Province Kings in Port Elizabeth on Tuesday (kick-off 7pm local time).
Warren Gatland's starting XV includes four uncapped players, with Matthew Morgan lining up at full-back, Steven Shingler at inside centre and Jordan Williams on the wing.
They are joined in the backline by Cardiff Blues duo Alex Cuthbert and Cory Allen as well as James Hook and Rhodri Williams at half back.
In the pack Paul James is named alongside Scott Baldwin and Rhodri Jones in the front-row with Jake Ball and Ian Evans in the second-row.
Captain Dan Lydiate is joined by new Cardiff Blues back row Josh Turnbull at flanker with Dan Baker at No 8.
On the bench Cardiff Blues hooker Matthew Rees is named in a Wales squad for the first time since February 2013.
Uncapped Gareth Davies is named amongst the replacements along with George North and Liam Williams.
WALES TEAM TO PLAY EASTERN PROVINCE KINGS:
Matthew Morgan (Ospreys), Alex Cuthbert (Cardiff Blues), Cory Allen (Cardiff Blues), Steven Shingler (Scarlets), Jordan Williams (Scarlets), James Hook (Perpignan), Rhodri Williams (Scarlets), Paul James (Bath), Scott Baldwin (Ospreys), Rhodri Jones (Scarlets), Jake Ball (Scarlets), Ian Evans (Ospreys), Dan Lydiate (Capt) (Racing Metro), Josh Turnbull (Cardiff Blues), Dan Baker (Ospreys).
Replacements: Matthew Rees (Cardiff Blues), Aaron Jarvis (Ospreys), Samson Lee (Scarlets), Luke Charteris (Perpignan), Aaron Shingler (Scarlets), Gareth Davies (Scarlets), George North (Northampton Saints), Liam Williams (Scarlets).
Supporters who have purchased tickets and are travelling to the European Rugby Challenge Cup clash with London Irish at the Madejski Stadium on Saturday (kick-off 3pm) are advised that some seating has now been changed for this fixture.
Supporters are advised that we will selling tickets right up until kick-off for the big home European Rugby Challenge Cup clash with London Irish - so don't miss all the action this Saturday at BT Sport Cardiff Arms Park.
Blues Rugby Camps will be back this February to given children right across our region the opportunity to participate in rugby during the holidays - and you can book NOW to avoid any disappointment and get the ideal Christmas gift for the aspiring young rugby player in your life!
A battling late fight-back failed to stop Cardiff Blues crashing to a disappointing derby day defeat as Newport Gwent Dragons held on to victory in a wet and blustery Guinness PRO12 encounter at BT Sport Cardiff Arms Park.