"It's important that we have a final look at certain players and combinations and this selection accommodates that," said Jenkins.
"It's the final opportunity for certain individuals to make a statement and after Saturday I'll be better placed to make the selection before the 14th of August."
Jenkins has selected his captain at inside centre partnering his Cardiff Blues colleague Tom Shanklin. Dafydd James, who missed Wales' tour of Australia through injury, is given a chance on the left wing and is joined by Aled Brew on the right. Lee Byrne fills the full back berth with Ceri Sweeney and Gareth Cooper forming the half back partnership.
Up front Will James is set to win the first cap of his career at the age of 30 and partners Cardiff Blues lock Rob Sidoli in the second row. Veteran back row Colin Charvis has passed a fitness test to be selected at flanker, Michael Owen is given his chance at No.8 and Alun-Wyn Jones completes the back row.
The front row is made up of Iestyn Thomas and the English-born Chris Horsman at prop with Huw Bennett at hooker. Tom James could also win his first cap if called up from the bench. Cardiff Blues front row forwards Gethin Jenkins and Rhys Thomas are also on the bench.
"Inside centre is one of the areas we are not absolutely clear about in terms of how our combinations are going to work, we are very comfortable with Gareth in that position and this is an excellent opportunity to give him another run there," said Jenkins.
"Gareth is well established and respected as a captain and the players will respond to him very well in Stephen's absence on Saturday.
"Up front it's the same story for us, again selection is about giving opportunity to players who didn't have a chance in the Six Nations.
"With Ryan Jones out Michael gets an opportunity at No 8 and Colin shows us how flexible and versatile an option he is by featuring at openside flanker.
"It was always going to be the case that the Australia tour would bring some players to the fore and that was the case for both of those players.
"Will (James) has impressed in training throughout the summer, he is not a young man at 30 but he is young in terms of rugby life, he has impressed everyone and has a real chance if he goes well on Saturday.
"We will be giving full respect to the tradition of this fixture and going out there looking to beat England and square the series.
"We hope to learn other things about our squad in the meantime but Wales teams have been travelling to Twickenham since 1910 and we want to join the other 51 sides that have notched up a 'W' against them."
England have achieved 9 successive victories at Twickenham, which matches the Welsh record at Cardiff Arms Park set between 1965 and 1981.
Results between the two sides currently give England a 52-51 lead with 12 matches drawn and Thomas, who earns his 95th cap could be the man to help Wales level the series.
Because of injuries that have been making people unavailable to training in the last few weeks Nicky Robinson and Robin Sowden-Taylor has also joined the camp to train with the squad.
WALES: Lee Byrne; Aled Brew, Tom Shanklin, Gareth Thomas (Captain), Dafydd James; Ceri Sweeney, Gareth Cooper; Iestyn Thomas, Huw Bennet, Chris Horsman, Will James, Rob Sidoli, Alun-Wyn Jones, Colin Charvis, Michael Owen
REPLACEMENTS: Gethin Jenkins, Rhys Thomas, TRThomas, Alix Popham, Mike Phillips, James Hook, Tom James
15 Lee BYRNE (OSPREYS)
DoB: 01.06.80. 6'3" / 1.91m. 15st 4lbs / 97kgs
International record: 13 caps (W 3, L 9, D 1; 10 pts - 2T)
14 Aled BREW (OSPREYS)
DoB: 09.08.86. 6.0" / 1.83m. 15st 7lbs / 98kgs
International record: 2 caps (L 2)
13 Tom SHANKLIN (CARDIFF BLUES)
DoB: 24.11.79. 6'2" / 1.88m. 15st 9lbs / 99kgs
International record: 40 caps (W 17, L 22, D 1; 85 pts - 17T)
12 Gareth THOMAS (CARDIFF BLUES, CAPTAIN)
DoB: 25.07.74. 6'2" / 1.88m. 16st 2lbs / 102kgs
International record: 94 caps (W 49, L 44, D 1; 190 pts - 38T)
11 Dafydd JAMES (LLANELLI SCARLETS)
DoB: 24.07.75. 6'3". 14st 5lbs
International record: 45 caps (W 21, L 23, D 1; 65 pts - 13T)
10 Ceri SWEENEY (NEWPORT GWENT DRAGONS)
DoB: 21.01.80. 5'10" / 1.78m. 13st 9lbs / 87kgs
International record: 31 caps (W 15, L 16; 84 pts - 4T, 23C, 5PG, 1DG)
9 Gareth COOPER (GLOUCESTER RUGBY)
DoB: 07.05.79. 5'7" / 1.71m. 13st 6lbs / 85kgs
International record: 33 caps (W 16, L 17; 30 pts - 6T)
3 Chris HORSMAN (WORCESTER WARRIORS)
DoB: 02.02.78. 5'11" / 1.81m. 17st 9lbs / 112kgs
International record: 10 caps (W 4, L 6; 5 pts - 1T))
1 Iestyn THOMAS (LLANELLI SCARLETS)
DoB: 15.12.76. 6'1". 18st 0lbs / 114kgs
International record: 32 caps (W 10, L 22)
2 Huw BENNETT (OSPREYS)
DoB: 11.06.83. 6'0" / 1.83m. 15st 10lbs / 100kgs
International record: 11 caps (W 6, L 5)
4 Will JAMES (GLOUCESTER RUGBY)
DoB: 22.12.76. 6'6" / 1.99m. 19st 2lbs / 122kgs
International record: Uncapped
5 Robert SIDOLI (CARDIFF BLUES)
DoB: 21.06.79. 6'6" / 1.99m. 18st 13lbs / 120kgs
International record: 41 caps (W 17, L 23, D 1; 10 pts - 2T)
6 Alun-Wyn JONES (OSPREYS)
DoB: 19.09.85. 6'5" / 1.96m. 18st 9lbs / 118kgs
International record: 10 caps (W 3, L 7)
7 Colin CHARVIS (NEWPORT GWENT DRAGONS)
DoB: 27.12.72. 6'2". 18st 0lbs / 114kgs
International record: 86 caps (W 39, L 45, D 2; 100 pts - 20T)
8 Michael OWEN (NEWPORT GWENT DRAGONS)
DoB: 07.11.80. 6'5" / 1.96m. 18st 2lbs / 115kgs
International record: 36 caps (W 17, L 18, D 1; 10 pts - 2T)
16 Gethin JENKINS (CARDIFF BLUES)
DoB: 17.11.80. 6'2" / 1.88m. 18st 9lbs / 118kgs
International record: 45 caps (W 17, L 26, D 2; 15 pts - 3T)
17 Thomas Rhys THOMAS (CARDIFF BLUES)
DoB: 23.04.82. 6'0" / 1.83m. 16st 8lbs / 105kgs
International record: 19 caps (W 8, L 10, D 1)
18 Rhys THOMAS (NEWPORT GWENT DRAGONS)
DoB: 31.07.82. 82. 6'1". 17st 6lbs
International record: 1 cap (L 1)
19 Alix POPHAM (LLANELLI SCARLETS)
DoB: 17.10.79. 6'2" / 1.88m. 17st 4lbs / 110kgs
International record: 24 caps (W 9, L 14, D 1; 15 pts - 3T)
20 Michael PHILLIPS (OSPREYS)
DoB: 29.08.82. 6'3" / 1.91m. 16st 6lbs / 104kgs
International record: 22 caps (W 10, L 11, D 1; 10 pts - 2T)
21 James HOOK (OSPREYS)
DoB: 27.06.85. 6'0" / 1.83m. 14st 3lbs / 90kgs
International record: 13 caps (W 3, L 9, D 1; 94 pts - 3T, 17C, 13PG, 2DG)
22 Tom JAMES (CARDIFF BLUES)
DoB: 17.04.87. 6'2" / 1.88m. 15st 1lb / 96kgs
International record: Uncapped