Attached coach Sophie Bennett said, "We are pleased with our position at the top of the table with maximum points from our first two matches. However, we won't be underestimating the strength of the Ospreys side.
"We have a good pack but we want to stick to our attacking style of rugby that has been successful so far and hopefully the boys will be well-supported in Merthyr."
Meanwhile, the Dragons and Blues South will be looking for their first win of the campaign at Cross Keys. Blues Under 16s North play Ospreys at Merthyr RFC (kick off 7:15pm).
Team: Callum Rowe, James Wood, Mitchell Dummett, Gavin Jones, Dafydd Hicks, Joshua Jones, Caine Rees, Garrick Legge, Callum Williams, Aaron Atkins, Thomas Payne, Kyle Evans, Richard Powell, Ieuan Davies (c), Chris Phillips
Replacements: Lloyd Peart Dean Gunter Will Rose Greg Morgan Neil Wallage Alex Pugsley Harri Gravell
Cardiff Blues Under 16s South travel to Cross Keys to take on the Dragons (kick off 7:15pm).
Team: Loren Quick, Rhys Slocombe, Ryan Healy, Ross Wardle, Dylan Jones, Gareth Llewellyn, Dan Fish, Gus Davies, Owain Thomas, Joe Poole (c), Adam Thompson, Daniel Jones, Alex Phillips, Declan Smith, Scott Gunter
Replacements: Michael Stephens, Dan Jones, Scott Mackie, James Llewellyn, Keith Machunga, Michael Walentine, Ashley Rowley