The game started at a fast pace with Blues exerting some good early pressure which led to the Ospreys giving away a penalty that Robyn Wilkins slotted over to make the score 3-0.
Ospreys hit back with a try to take the lead, but Wilkins added two kicks to put her side back ahead before a late score from Laurie Harries secured the win and title.
Head Coach Nadine Griffiths said: "It has been an honour and pleasure to coach the Blues Women this year. Their commitment on and off the field has been outstanding which showed itself on the pitch in the final game with Ospreys. There has been a great level of skill and talent on display which I'm sure will cause the national coaches a few headaches in selection."
Blues Women: Gemma Hallett (c), Laura Prosser (v/c), Aimee Dymek, Lily Snape, Nia Jones, Kim Jones, Beth Kenure, Sara Prosser, Jenny Hawkins, Anna Jenkins, Kristen Fairbairn, Catrina Nicholas, Becky Newton, Sioned Davies, Laura Prosser, Laura Shanahan, Robyn Wilkins, Jade Newman, Elin Huxtable, Laurie Harries, Kat Lenon, Chantelle Miller, Cerys Ballet, Rafuike Taylor, Philippa Tuttiet, Kath Salter, Kate Hughson.
Coaches: Nadine Griffiths, Head Coach; Liam Scott, Forwards Coach; Dan Clements & Mike Morgan, Backs Coaches; Ray Gallivan, Team Manager.
The Wales Women's squad has now been selected and congratulations to the Blues players who have been selected byHead Coach Rhys Edwards for the 2014 Six Nations Championship. They are Jenny Davies, Nia Davies, Jade Newman, Catrina Nicholas, Laura Prosser, Robyn Wilkins, Philippa Tuttiett and Laurie Harries.